Identification of a spectrin-like protein in nonerythroid cells.
نویسندگان
چکیده
منابع مشابه
Endothelial cells express a spectrin-like cytoskeletal protein.
Vascular endothelium was investigated by indirect immunofluorescence and immunoautoradiography for the possible presence of spectrin-like molecules. Antibodies were raised against electrophoretically purified rat, rabbit, and bovine red blood cell spectrin and against rabbit brain fodrin. Antibody specificity was assessed by immunoblotting and double-diffusion technique. Homogenates of endothel...
متن کاملBRIEF COMMUNICATIONS Endothelial Cells Express a Spectrin-like Cytoskeletal Protein
Vascular endothelium was investigated by indirect immunofluorescence and immunoautoradiography for the possible presence of spectrin-like molecules. Antibodies were raised against electrophoretically purified rat, rabbit, and bovine red blood cell spectrin and against rabbit brain fodrin. Antibody specificity was assessed by immunoblotting and double-diffusion technique. Homogenates of endothel...
متن کاملCrystal structure of the nonerythroid alpha-spectrin tetramerization site reveals differences between erythroid and nonerythroid spectrin tetramer formation.
We have solved the crystal structure of a segment of nonerythroid alpha-spectrin (alphaII) consisting of the first 147 residues to a resolution of 2.3 A. We find that the structure of this segment is generally similar to a corresponding segment from erythroid alpha-spectrin (alphaI) but exhibits unique differences with functional significance. Specific features include the following: (i) an irr...
متن کاملTissue-specific expression of distinct spectrin and ankyrin transcripts in erythroid and nonerythroid cells
cDNA probes for three components of the erythroid membrane skeleton, alpha spectrin, beta spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a lambda gt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)+ RNA. Each cDNA appears to hybridize to one gene type in the chicken genome. Qualitatively distinct RNA species in myogenic and ery...
متن کاملTissue-specific Expression of Distinct Spectrin and Transcripts in Erythroid and Nonerythroid Cells Ankyrin
cDNA probes for three components of the erythroid membrane skeleton, a spectrin,/3 spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a Agtl 1 expression vector library containing cDNA prepared from chicken erythroid poly(A) + RNA. Each cDNA appears to hybridize to one gene type in the chicken genome. Qualitatively distinct RNA species in myogenic and erythroid cell...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1981
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.78.12.7570